How to handle and store my siRNA oligo?
Storage:
- siRNA oligos are shipped as dry pellets at room temperature, Under these condition, they are stable for at least four weeks.
- Upon receipt, siRNA reagents should be stored at -20OC to -80OC. Under these conditions, they are stable for at least one year.
- siRNA should be resuspended in RNase-free solutions. RNase-free water (for short-term storage) is appropriate for resuspension of concentrated stocks (i.e. 20-100 µM). Alternatively, an RNase-free buffer (pH7.3-7.6) maybe used such as PBS.
Handling Precautions:
Oligonucleotides are susceptible to enzymatic degradation by nucleases and to chemical degradation by extreme pH and temperature. We recommend wearing gloves and maintaining nuclease-free conditions when handling the oligonucleotides.
Print
12/30/2008