Enhanced Diagnostic Tools
A thiol group can be used to attach an oligo to a variety of fluorescent and nonfluorescent moieties or surfaces. Oligos containing thiol modifiers are shipped in their oxidized (disulfide) form and require chemical reduction by dithiothreitol (DTT) or Tris (2-carboxyethyl) phosphine (TCEP) prior to use.
5' Thiol Modification:
3' Thiol Modification: Thiol Modifier C3 S-S (Disulfide). Oligonucleotides with the 3'-Thiol Modifier C3 S-S (Disulfide) will need to be reduced before use.
Procedure for 5'-Thiol-Modifer C6 Oligonucleotides (5'-Trityl-S-(CH2)6-phosphodiester bond-oligonucleotide-3')
Procedure for 5'-Thiol-Modifier C6 SS and 3'-Thiol-Modifier C3 SS Oligonucleotides
(5'-(CH2)6-S-S-(CH2)6-phosphodiester Bond-Oligonucleotide-3') or (5'-Oligonucleotide-phosphodiester bond-(CH2)3-S-S-(CH2)3-OH-3')
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