Enhanced Diagnostic Tools
Petide carrier protein conjugation is an critical step in generating anti-peptide antibodies. Finding the best coupling strategy to cross-link peptides to an appropriate carrier molecule is often overlook during peptide designing stage. Selecting the most appropriate carrier protein and choosing the correct conjugation chemistry can enormously improve the chance of producing high titre antibodies towards your antigen of interest. Peptides used for antiserum production require conjugation to a carrier since the peptide alone is usually insufficient in generating an immune response. Selection of a coupling strategy becomes extremely important, since the conjugated immunogen should present an epitope similar to that observed in the native protein, with the correct orientation and flexibility. Alternatively, use the MAP strategy for synthesis of the peptide (not suitable for peptides from the c-terminus) can be used for generating antibody.
There are 3 items to consider when determining peptide conjugation
Steps for choosing conjugation chemistry:
Location of peptide segment within the native protein:
Multiple Antigen Peptide (MAP)
MAP is simply an alternative to protein conjugation. It is recommended only for shorter peptide located either internally.
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