How to Select a Peptide Synthesis Service Provider ?
Synthetic oligo-peptides are extensively used in research of structure and function analysis (for example to study protein-protein interfaces) for the development of binding assays, the study of receptor agonist/antagonists or as immunogens for the production of specific antibodies. Generally, peptides are synthesized by coupling the carboxyl group or C-terminus of one amino acid to the amino group or N-terminus of another using automated solid phase peptide synthesis chemistries. However, liquid phase synthesis maybe used as well if the need arises.
Several important factors must be considered when selecting a peptide synthesis service provider. The quality and the length limit of the synthesized peptides will determine how good the service providers are since it is more difficult to synthesize longer peptides at high quality. It is very important to consider the quality control (QC) methods and the availability of QC reports when selecting a provider. QC is usually done by analytical HPLC and mass spectrometry. Additionaly, amino acid analysis and sequencing can also be requested.
Other important features one should look for when selecting a provider are the flexibility of the company regarding the quantity of the peptides they can synthesize. Furthermore, one should evaluate the synthesis of linear or cyclic peptides of various lengths as well as the ability to provide modifications such as acetylation, amidation, biotinylation, phosphorylation, disulfide bridge formation (-S-S- cyclization), fluorescein, myristylation, phosphorylation, rhodamine to the peptides available. Another factor to consider is the incorporation of non-standard residue or the use of pseudo-prolines.
Biosynthesis Inc. is able to fulfill the above criteria and offer competitive pricing, fast delivery and ease of ordering. Furthermore, in order to obtain the best experimental results for your specific application, the staff at Biosynthesis Inc. offers technical support from the design phase to the selection phase of peptide modifications.
Print
11/06/2013