Does 3' spacer modification portect against exonuclease and polymerase activity?
There is no definitive data on the assertion that 3' spacer modification will protect oligos from exonuclease digestion and does not permit polymerase extension. Our conclusion is based by analogy to the propylamino-modifier This modification protects oligos from exonuclease digestion but permits polymerase extension to a small extent since the modifier is eliminated to a level of about 10% from the 3' terminus, leaving the 3'-hydroxyl group available. HPLC experiments have shown that there is no detectable elimination of the propyl group from oligos made from the spacer C3.
REFERENCE(S):
- J.G. Zendegui, K.M. Vasquez, J.H. Tinsley, D.J. Kessler, and M.E. Hogan, Nucleic Acids Research, 1992, 20, 307-314.
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11/06/2013