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Typical Buffers Used In BNA Experiments

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Typical buffers used in BNA experiments

Application
Concentration
Buffer
Reference
 
 
 
 
Antisense BNAs
1 micomolar (1 µM)
20 mM sodium phosphate, 100 mM NaCl pH 7.2
Obika et al., 2001
Duplex formation
Duplex concentration 4 micromolar (µM)
100 mM NaCl, 10 mM sodium phosphate pH 7.2
Obika et al., 1998
Duplex formation
4 microM (µM) per strand
100 mM NaCl, 10 mM sodium phosphate pH 7.2
Imanishi and Obika 2002
Duplex formation
4 micromolar (µM)
100 mM NaCl, 10 mM sodium phosphate pH 7.2
Miyashita et al., 2007
Triplex formation
> 0.2 µM
140 mM KCl, 10 mM MgCl2, 7 mM sodium phosphate pH 7.0
50 mM Tris-acetate, 100 mM MgCl2 pH 7.0
10 mM sodium cacodylate/cacodylic acid pH 6.8 200 mM NaCl, 20 mM MgCl2
Hari et al., 2003
 
Torigoe et al., 2011
RNAi
1 µM
Transfection:
0.2 to 20 nM
100 mM NaCl, 10 mM sodium phosphate pH 7.2
Transfection: Lipofectamine 2000 in OPTI-MEM buffer
Rahman et al. 2010,
Yamamoto et al. 2012