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What buffer components can I use with my antigen?

What buffer components can I use with my antigen?

We cannot accept immunogens in buffers that have any of the following conditions (for immunizations):

  • Contain ANY metal dyes
  • Contain KCl, NaCl, or MgCl2 at > 1.0 M
  • 5% SDS (w/o consulting our veterinarian)
  • 6M Urea (w/o consulting our veterinarian)
  • 1% b-octylglucoside, a detergent
  • 1% Triton 100, a detergent
  • 1% Tween 20, a detergent
  • > 20% glycerol
  • 0.1mM PMSF (Protease Enzyme inhibitor)
  • 1ug/ml Leupeptin (Protease Enzyme inhibitor)
  • 1ug/ml Pepstatin A (Protease Enzyme inhibitor)
  • 0.1mM DIPF (Protease Enzyme inhibitor)
  • 3M DTT (a reducing agent)
  • 3M imidizole