The OD260nm (Optical Density) value denotes the amount of oligonucleotide you have received. The conversion of OD260nm values to nanomoles depends on the molecular weight of the individual oligo. Therefore no general applicable conversions can be offered.
1 OD260nm |
Equals |
Conversion - nmole |
30 µg ss-DNA |
4.5 nmol of a 20mer |
40 µg ss-RNA |
1.8 nmol of a 50mer |
50 µg ds-DNA |
0.9 nmol of a 100mer |
* only valid for ss-oligos with equal base composition of all four bases.