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What is a TaqMan MGB probe?

MGB stands for minor groove binder. It is a non-fluorescent quencher that increases the Tm of a probe, because of its minor groove binding ability. Therefore, MGB probes are about 13 to18 nucleotides long, shorter than other types of probes.

Alternative MGB probes will be the use of BNA, which offers better hybridization selectivity. Learn more about the MBG probe BNA alternative.