Enhanced Diagnostic Tools
The crude oligo will contain full length product and n-1 failure sequences. Our Tom synthesis chemistry has very high coupling efficiencies, resulting in ~ 99% incorporation at each step. Any growing oligo that does not receive the next base in the sequence is capped and thus prevented from 'growing' incorrectly. Thus, the full length product will have the complete correct sequence and any shorter fragments will have complete sequence fidelity, but represent truncated oligos.
The percent of full length product is calculated by: 0.99exp(n), where 0.99 = the coupling efficiency and n = the number of couplings (length of the oligo - 1). For example, for a 21-mer, the calculation is 0.99exp20 = 0.82, or 82% full length material with the rest representing truncation products.
If you have a query regarding any of our products or services,
please don't hesitate to submit your question below. A member of our support team
will review and answer your question as quickly as possible.