Enhanced Diagnostic Tools
Oligonucleotide with 3' glyceryl modification can be oxidized by sodium periodate to form 3' phosphoglycaldehyde. This aldehyde can be further oxidized to the corresponding carboxylic acid. Either the aldehyde or the carboxylate may then be used for subsequent conjugation to Amine-containing products.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
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