Halogenated Base Oligo Modification
Custom DNA Synthesis--"FASTEST services in the industry"
Halogenated Bases
Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide
structure. They are also photolabile and are used for cross-linking studies to probe
the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides
containing Br-dU can be used as probes.
Click on specification page for details.
|
br-dU
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$100
|
$100
|
$80
|
|
1.0 µmol
|
35 OD
|
$160
|
$160
|
$150
|
|
5-Br-dC
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$160
|
$160
|
NA
|
|
1.0 µmol
|
35 OD
|
$160
|
$160
|
NA
|
|
8-Br-dA
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$280
|
$280
|
NA
|
|
1.0 µmol
|
35 OD
|
$280
|
$280
|
NA
|
|
8-Br-dG
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$200
|
$200
|
$80
|
|
1.0 µmol
|
35 OD
|
$200
|
$200
|
$95
|
|
5-I-dC
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$340
|
$340
|
NA
|
|
1.0 µmol
|
35 OD
|
$340
|
$340
|
NA
|
|
5-I-dU
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$160
|
$160
|
NA
|
|
1.0 µmol
|
35 OD
|
$160
|
$160
|
NA
|
|
5-F-dU
|
Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
200 nmol
|
15 OD
|
$340
|
$340
|
NA
|
|
1.0 µmol
|
35 OD
|
$340
|
$340
|
NA
|
Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Quality Controls
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.
The most trusted name in Quality Custom Synthesis