Enhanced Diagnostic Tools
The selective recongintion of nucleic acid base sequences is critical in molecular detection and therapeutic system. While gene expression is controled by sequence-specific regulatory proteins (repressor or activators) which shown to recognize the major groove of the DNA, however, many DNA-binding drugs often bind to the minor groove. Intercalating modified oligonucleotide are positively charged polycyclic aromatic molecules, which usually bind between two consecutive base pair of double-helical DNA via the minor groove. Thermodynamic studies have shown that intercalator modified oligonucleotide enhance binding specificity and stability due to the additional binding energy provided by the intercaltor. Short oligonucletoides can thereby reach a binding strength under physiological conditions that is sufficint to elicit a biological response. Intercalating modified oligonucleotides also made to resistant nuclease. These oligo modification often used extensively in the study of DNA structure and mutagenic, antiviral, antimitotic, anticarcinogenic,antimalaria activity
Contact Bio-Synthesis for triplex forming Intercalator modified oligonucleotide conjugates.
Bio-Synthesis intercalator bases can be incorporate at any position of an oligonucleotide . Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.