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| Bio-Synthesis continued to provide quality DNA products and services for the research community, but it has also become a world leader in providing custom peptide products and services. Using state-of-the-art solid phase peptide chemistries, molecular-based PNA FISH tests that provide rapid identification of bloodstream pathogens in hours instead of days, enabling labs to quickly report critical information to physicians and pharmacists to ensure optimal therapy and help reduce death rates for patients afflicted with bloodstream infections. |
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Peptide Selection and Design |
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The first step in the process is the selection of the appropriate peptide sequence. At this step the ultimate use for the antibody must be considered. If the antibody is needed to probe a specific protein domain then the choice is simple. |
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Coupling Strategy |
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A factor that is often over-looked when designing a synthetic peptide is the method of coupling the peptide to the carrier protein. For example, N-terminal sequences should be coupled through the C-terminal amino acid and vice versa for C-terminal sequences. |
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Selecting the Protein Carrier |
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Conjugation to a carrier protein is important because peptides are small molecules, which alone do not tend to be immunogenic, thus possibly eliciting a weak immune response. The carrier protein contains many epitopes that stimulate T-helper cells, which help induce the B-cell response. |
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