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| For over 20 years, BioSynthesis has provided custom antibody production services worldwide, including researcher at university, biotechnology and pharmaceutical institutions. We offer comprenhensive services for polyclonal antibody production with large selection fo host animals. Each of our facilities is operated under USDA license and hold an NIH Animal Welfare Assurance from the Office of Laboratory Animal Welfare. |
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Hybridization Probe |
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In molecular biology, a hybridization probe is a fragment of DNA of variable length (usually 100-1000 bases long), which is used to detect in DNA or RNA samples the presence of nucleotide sequences that are complementary to the sequence in the probe. The hybridization probe is labelled radioactively (commonly with 32P) or with immunological markers, such as digoxigenin. |
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Labelled Probe |
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The labelled probe is then denatured (by heating) into single DNA strands and hybridized to target DNA (Southern blotting) or RNA (northern blotting) immobilized on a membrane or in situ. DNA sequences or RNA transcripts that have moderate to high (depending on the stringency in the hybridization) sequence similarity to the probe are detected by visualizing the hybridized probe via autoradiography or other imaging techniques. Hybridization probes used in DNA refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, and to which a mobile DNA target is hybridized. |
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