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Polyclonal antibodies were developed for the detection of Sp17 in human tissues. A recombinant Sp17 protein was expressed and purified for use as an antigen. Polyclonal anti-sera was harvested after seven immunizations with the Sp17 recombinant protein. ELISA analysis detected positive anti-recombinant Sp17 titers as low as 1:500 dilution of the rabbit anti-sera. Furthermore, dot blot analysis revealed that the polyclonal anti-Sp17 sera exhibited high affinity for concentrated Sp17 recombinant protein.
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