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Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss

Laura Maskin; Nicola´s Frankel; Gustavo Gudesblat; Marı´a J. Demergasso; Lı´a I. Pietrasanta; Norberto D. Iusem
11/30/2013
ScienceDirect

Asrgene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that In vitro , electrophoretic assays show a homo-dimeric structure for ASR1 and highlight strong non-covalent interactions tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss.between monomers prone to self-assemble. Direct visualization of single molecules by atomic force microscopy (AFM) confirms that ASR1 forms homodimers and that uncovers both monomers and dimers bind double stranded DNA. The