Double-Stranded DNA-Templated Oligonucleotide Digestion Triggered by Triplex Formation
Satoshi Obika, et al.
11/28/2013
DNA-templated organic synthesis (DTS)[1] has attracted a great deal of attention in the fields of organic, bioorganic, and analytical chemistry. Conventional DTS involves hybridization of two oligonucleotide probes with each other[2] or with a template- strand,[3, 4] followed by a bond-forming reaction between reactive substituents conjugated on each strand. Several methodologies for sequence-specific DNA and RNA detection have been developed based on conventional DTS.[5–11] Examples ACHTUNGTRENUNGinclude PNA-based ligation probes[6–8] and quenched autoligation probes.[9–11] The PNA-based ligation probe exhibited high mismatch selectivity,[6, 7] and quenched autoligation probes, coupled with fluorescence resonance energy transfer (FRET) technology, have been used to do imaging in living cells.[10,11] Bond cleavage reactions promoted by hybridization with DNA templates comprise a new class of DTS and should be useful for novel DNA sensing technologies. In fact, successful results have been reported by Mokhir et al[12] and Taylor et al.[13] These approaches will likely prove advantageous in improving the sensitivity of DNA or RNA detection due to catalytic turnover.