Enhanced Diagnostic Tools
Bio-Synthesis offers metal chelate oligo modification services. These chelate-ligand modified oligonucleotides are suitable for in vivo siRNA imaging,1 or sequence selective DNA cleavage.2 Researchers have prepared metallo-DNA supramolecular assemblies that raise the fascinating possibility of metal-based molecular nano-devices such as molecular scale magnets and wires.3,4 Metallobases can be used to precisely control modified DNA through the addition (or removal) of a small amount of selected metal ion, which may lead to astonishing advancements in oligonucleotide research. Several groups have already reported exceptionally stable unnatural metallobase pairs using ligand modified oligonucleotides.5-8
Oligo metal-ligand labeling can be incororated into an oligonucleotide at the time of synthesis or conjugate post-synthetically via various type of cross-linking chemistry. Post-synthetic chemical modifications made to an oligonucleotide require dual HPLC purification.
Contact Bio-Synthesis for metal chelate oligo modifications.
Bio-Synthesis metal chelate conjugates can be incorporate at any position of an oligonucleotide. Dual HPLC purification is required. Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.