Enhanced Diagnostic Tools
Standard "wobbles" are equimolar mixtures of two or more different bases at a given
position within an oligonucleotide. Oligos with wobbles can be used as probes to
hybridize to an unknown gene that encodes a known protein sequence, for random mutagenesis,
and combinatorial chemistry.
Inosine occurs naturally in the wobble position of the anticodon of some transfer
RNAs and is known to form base pairs with A, C and U during the translation process.
Please always refer to the International Union of Biochemistry (IUB) base codes
to introduce wobbles in your oligo sequences..Click on specification page for details.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.