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Mix Bases

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DNA Modifications

Degenerate bases (Wobbles)

Standard "wobbles" are equimolar mixtures of two or more different bases at a given position within an oligonucleotide. Oligos with wobbles can be used as probes to hybridize to an unknown gene that encodes a known protein sequence, for random mutagenesis, and combinatorial chemistry.

Inosine occurs naturally in the wobble position of the anticodon of some transfer RNAs and is known to form base pairs with A, C and U during the translation process.

Please always refer to the International Union of Biochemistry (IUB) base codes to introduce wobbles in your oligo sequences..Click on specification page for details.

Inosine dI Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $30 $30 $30
1.0 µmol 35 OD $35 $35 $35
dA+dG (R) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dC+dT (Y) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA + dC (M) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dG + dT (K) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dC + dG (S) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA + dT (W) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA + dC + dT (H) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dC + dG + dT (B) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA + dC + dG (V) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA +dG + dT (D) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12
dA+dC+dG+dT (N) Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD $5 $5 $10
1.0 µmol 35 OD $8 $8 $12

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us.

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