Oligo Labeling for Non-Radioactive Hybridization Probes
In spite of significant advances in the detection of fluorescent and luminescent labels, oligo-enzyme conjugates continue to be the most sensitive reporter groups. For most applications, direct conjugation of enzyme to oligonucleotide offer the best overall performance with the highest sensitivity, specificity, simplicity and rapid detection. Nucleic acid enzyme conjugates eliminate the need for radioactivity in most assay systems.
Bio-Synthesis now offers custom Oligo-Enzyme conjugation services including:
- Oligo synthesis, functionalization, purification and activation of oligo up to 25 bases long
- Conjugation of oligo to select enzyme of choice
- Validated by mass spectrometry and SDS gel electrophoresis
DNA or RNA can be conjugated to small molecules such as:
- Horseradish Peroxidase (HP)
- Alkaline Phosphatase (ALP)
- Alkaline Phosphatase Amplification (AMP)
- Penicillinase (PNC)
- Streptavidin
- Biotin-Avidin
- Alpha-D-Galactorsidse
- Glucose-t-Phosphate Dehydrogenase
- Other Enzyme Innunoconjugates
- Non-enzyme proteins