Enhanced Diagnostic Tools
The stability of the RNA-DNA duplex in terms of hybridization and half-life is crucial to successful gene inhibition. Synthetic oligonucletoides used in cell culture or in vivo experiments often face the chanllenge of degradation as well as chemical instability which often lead to fast degradation with a finite half life1. While unmodified DNA and RNA oligos are quickly digested in vitro and in vivo by multiple endo- and exonuceases2, vigorous research activity in the area of nucleic acid chemistry has been devoted in developing novel base analogs to block nuclease degradation, at same time, retain its molecular structure and base interaction. This crucial property is indispensable for gene silencing experiments - such as in antisense, RNAi and in ribozyme technology. To limit nuclease sensitivity and enhancing intracellular statbility, many different oligo modifications into native phosphodiester oligodeoxyribonucleotide and ribonucleotide polymers are available
Bio-Synthesis offers an extensive array of modifications to accomplish duplex stability and nuclease resistance to synthetic oligos. We have the ability to synthesize complex combinations of modifications and chimeric oligos. In addition to the synthesis of these modified oligos, we routinely assist customers in the design of the oligos that are particularly suited to their application. Common modification sites are:
Contact Bio-Synthesis for oligo modification to block nuclease degradation.
All oligonucleotide synthesized at Bio-Synthesis is quality check by using either MALDI-TOF mass spectrometry, analytical HPLC,or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform custom formulation and quality control tailored to meet your specific requirements.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.