Oligo Modification for DNA Damage and Repair

Modified bases for DNA damage/repair studies

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

8-Oxo-dA	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$135	$135	NA
1.0 µmol	35 OD	$335	$335	NA
8-Oxo-dG	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$177	$177	NA
1.0 µmol	35 OD	$335	$335	NA
5-OH-dC	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$275	$275	NA
1.0 µmol	35 OD	$775	$775	NA
5-OH-dU	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$225	$225	NA
1.0 µmol	35 OD	$675	$675	NA
5-Hydroxymethyl-dU	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$225	$225	NA
1.0 µmol	35 OD	$675	$675	NA
1-Me-dA	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$125	$125	NA
1.0 µmol	35 OD	$250	$250	NA
N3-Cyanoethyl-dT	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$200	$200	NA
1.0 µmol	35 OD	$600	$600	NA
5,6-Dihydro-dU	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$195	$195	NA
1.0 µmol	35 OD	$595	$595	NA
Pyrene-dU	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$105	$105	NA
1.0 µmol	35 OD	$210	$210	NA
Perylene-dU	Qty.	5'-Mod	Internal	3' -mod
200 nmol	15 OD	$150	$150	NA
1.0 µmol	35 OD	$300	$300	NA

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us.