Enhanced Diagnostic Tools
Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals,
and by ultraviolet and ionizing radiation. The body has therefore evolved a number
of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers
allow investigation of the structure and activity of oligonucleotides containing
an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions
or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds
that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy
pyrimidines are major base damage products formed by exposure of DNA to ionizing
Click on specification
page for details.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.