2-Fluoro deoxy Inosine dl (convertible dG) Oligonucleotide Modification
2-Fluoro-deoxyinosine (2-F-dI), a convertible dG minor base. 2-Fluro dI modified oligo react with a primary amine and displaces the fluorine atom, and converts the nucleotide into a N2-substituted dG in DNA for structral studies. Oligos modified with 2-F-dI are often use as precursors in the studies of requiring cross-linking, at G position(s), between oligos, or between an oligo and an enzyme.
2-F-dI modified oligos can react with disulfide-containing diamines (1) or thiopropylamines (2) in order to subsequently form disulfide-crosslinked DNA duplexes. Such oligos have also been reacted with bis-(3-aminopropyl)disulfide dihydrochloride, and the disulfide-containing oligo intermediate coupled to a short-lived HIV-1 reverse transcriptase kinetic intermediate to form stable enzyme-oligo complexes. The ability to synthesize such complexes have enabled deeper study of the DNA translocation mechanism of HIV-1 RT (3).
In order to minimize the unwanted side reactions with the exocyclic amines of other bases of the oligo, it must be fully protected and still attached to the synthesis solid support when reacted with the primary amine. As a result, all 2-fluoro-deoxyinosine modified oligonucleotide are deliver in protected form for subsequent conversion to N2-modified dG by customer. Contact Bio-Synthesis to order 2-Fluoro deoxy inosine oligonucleotide modification.
Product Information
2-Fluoro deoxy Inosine dl (convertible dG) Oligonucleotide Modification
Minor base, cross linked base
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
References
- Erlanson, D.A.; Chen, L.; Verdine, G.L. DNA Methylation through a Locally Unpaired Intermediate. J. Am. Chem. Soc. (1993), 115: 12583-12584.
- Erlanson, D.A.; Glover, J.N.M.; Verdine, G.L. Disulfide Cross-linking as a Mechanistic Probe for the B<-->Z Transition in DNA. J. Am. Chem. Soc. (1997), 119: 6927-6928.
- Sarafianos, S.G.; et al. Trapping HIV-1 Reverse Transcriptase Before and After Translocation on DNA. J. Biol. Chem. (2003), 278: 16280-16288. - 2-Fluoro deoxy inosine dI (convertible dG)
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