7-deaza-8-aza-dG DNA Modification
7-deaza-8-aza dG is a modified DNA base that has been used in analyzing DNA segment that are rich in G content. In DNA research, DNA base pairing are disrupted in G-rich segments becauseof their ability to create inter- and intra- strand hydrogen bonding. This aggregation causes enzymatic disruption so sequencing and PCR experiments become highly problematic. In probe experiments, these segments are not accessible due to this aggregation of G residues on the probe and/or target. The problem arises from extra hydrogen bonding forming at the N7 position of G1 . The substitution of base G with 7-deaza-8-aza-dG (pyrazolo[3,4-d]pyrimidine) (PPG) for G were evaluated by Seela's group where the N7 and C8 atom of the G base are flipped while allowing the modified base to retain the same electron density as the guanine ring system.
The 7-deaza-8-aza-G - C base pair was found to be stable relative to G - C by around 1° per insertion. This stability enhancement has led to interest in the use of PPG in diagnostic probe applications.
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7-deaza-8-aza-dG DNA Modification
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7-deaza-8-aza-dG DNA Modification
2' Deoxyribonucleoside analogs
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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