Bio-Synthesis offers Alexa 750 fluorescent dye oligonucleotide labeling services. Alexa 750 is a near infrad-red dye long wavelength dye With a peak excitation at 749 nm, oligo conjugates of Alexa Fluor® 750 dye are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. Alexa Fluor® 750 is an alternative dye for Cy7. This dye exhibits fluorescence spectra that are very similar to those of Cy7 dye. Its fluorescence emission maximum at 775 nm is well separated from commonly used far-red fluorophores such as Alexa Fluor® 647, Alexa Fluor® 660 or allophycocyanin (APC), facilitating multicolor analysis.
Alexa 750 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Aelxa 750 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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