Thymidine Glycol Oligonucleotide Modification
Thymidine gylycol (5,6-dihydroxy-5,6-dihydrothymine) can be incorporate at any position within an oligonucleotides. Thymine glycol (5,6-dihydroxy-5,6-dihydrothymine) (1) is formed when thymine is subjected to oxidative stress, including ionizing radiation or other oxidative mutagens. Oxidation of the 5,6 double bond of Thymidine generates two chiral centers at C5 and C6. The cis-5R,6S form (1) is generated as the predominant product along with the other diastereomer, the cis-5S,6R form (2). The presence of thymidine glycol in DNA has significant biological consequences and many organisms possess specific repair enzymes for the excision of this lesion. Thymidine glycol modified base can be incorporate at the time of synthesis. Ultamild synthesis chemistry is required to prepare these modified oligonucleotides.
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Thymidine Glycol Oligonucleotide Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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