Bio-Synthesis offers Alexa 350 fluorescent dye oligonucleotide labeling services. Alexa 350 is a blue-fluorescent dye has moderate photostability and excitation that meaches the 350 nm laser line. Alexa 350 has the brightness and photostability of blue dyes that are highly suitable for direct imaging and high-abundance targets like cytoskeleton markers. Commonly used with Alexa Fluor® 488, 594, and 647 dyes for multiplexing.
The fluorescence conjugates has peak excitation wavelength in 346 nm and emission wavelength in 442 nm and the bright blue fluorescence at a wavelength slightly shoter than that of AMCA. This dye has spectral overlap witht the emission of fluorecein or Alexa 488 dye.
Alexa 350 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 350 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.