Bio-Synthesis offers Alexa 594 fluorescent dye oligonucleotide labeling services. Alexa 594 is a red fluorescent dye that can be excited using 561 nm or 594 nm laswer lines. This dye is pH-insensitive over a wide molar range which permit stable signals for imaging and flow cytometry application. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. this dye can be use to replace Texas Red, it can be use to detect low abundance biological structure especially in fixed cells. It is commonly used with Alexa Fluor® 350, 488, and 647 dyes for multiplexing. In addition, is used in a range of SRM applications including dSTORM, SIM, and STED and in two-photon excitation (TPE) microscopy. Alexa Fluor® 594 is an alternative dye for Texas Red. This molecules can be attached to oligo at multiple sites at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
Alexa 594 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 594 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.