Bio-Synthesis offers ATTO 590 fluorescent dye oligonucleotide labeling at any position of an oligonucleotide. ATTO590 is a class of Rhodamine fluorecent dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 590 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. Features:
ATTO 590 is consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on hte request dye can also be supplied in isomeric purity. The fluorescence is excited most efficiently in the range 575-610 nm. This dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide. Contact us for ATTO 590 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.