ATTO MB2 Redox-dye Oligonucleotide Labeling
Bio-Synthesis offers ATTO MB2 Redox dye oligonucleotide labeling at any position of an oligonucleotide. ATTO MB2, a derivative of Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation (e.g. with oxygen) the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient. The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
ATTO 514 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for ATTO MB2 fluorescent dye oligonucleotide labeling services.
Product Information
ATTO MB2 Redox-dye Oligonucleotide Labeling
Fluorescent dye Label, Redox Labels
Extinction Coefficient, ε(λ):
110,000
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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