Maleimide modified oligonucleotides are typically used to form conjugates with thiol-labeled moieties, or Diels-Alder cycloaddition products. Maleimide can be incorporate during oligo synthesis using phosphoramidite chemistry at 5' end separated oligo with 2 carbon spacer arm. Maleimide modified oligo can be also incorporate post-synthetically which allow longer spacer in between functional group and oligonucleotide. We normally provides the oligonucleotide in lyophilized form in ready-to-use or protected state. Deprotection can be easily deprotected by Retro Diels-Alder reaction prior use. The Retro Diels-Alder reactions involves dehydration by co-evaporation with anhydrous acetonitrile and anhydrous toluene. The Retro Diels-Alder reaction requires anhydrous conditions and any significant level of moisture can cause incomplete deprotection, hydrolysis, and/or addition of water to the maleimide. The evaporation of the toluene leaves a white residue ready for conjugation. A detailed procedure is shipped with the product. An abbreviated procedure is described below.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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