Enhanced Diagnostic Tools
Oyster fluorophores is new class of cyanine dyes which can be use to substitute for Cy2, Cy3, Alexa or Fluorescein for FISH application. These dyes are unique in that they are:
This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for internal labeling, a double HPLC purification is required.
Contact your Account Representative for pricing on other dyes dye labeled oligonucleotides. For additional information, please Contact us.
Price/labeling, purification and synthesis is an additional charge
A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.