Phosphorylated Oligonucleotides
Bio-Synthesis offers phosphorylated oligonucleotide synthesis at various scales and purities. Chemically synthesized oligonucleotides are not phosphorylated at their either end. However, phosphorylation might be needed to mimic nucleic acid structure for various biological applications. 5' Phosphorylation is strictly required for the direct ligation to a vector without prior use of T4 polynucleotide kinase. While 3’ phosphorylation will inhibit degradation by some 3'-exonucleses and used to block extension by DNA polymerase. Both 3' and 5' -phosphorylated oligonucleotides are been used for either further chemical modification or as a substrate in DNA repair studies. Chemical phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.
Contact Bio-Synthesis for Phosphorylated Oligonucleotide Service.
Phosphorylation of Oligonucleotides
5 ́- and 3 ́- phosphorylation is available at all synthesis scales from 100 nmol up to 10 μmol.
Phosphate [Phos]
Terminal Phosphate Oligo Modification
Price
Price/modification. 10%-15% discount for order more than two oligo at same time.
Purification :
Phosphorylation requires additional processing in order to ensure high quality and yield. Purification by PAGE is recommended for long oligonucleotide, extended HPLC is also available.
Quality Controls:
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
| 5' Phosphoate |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$50.00
$50.00
$100.00
$280.00
$540.00
$50.00
$50.00
$100.00
$280.00
$540.00
|
|
| 3' Phosphoate |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$50.00
$50.00
$100.00
$280.00
$540.00
$50.00
$50.00
$100.00
$280.00
$540.00
|
|
Thiophosphate [TPhos]
Terminal Phosphoate Oligo Modification
Price
Price/modification. 10%-15% discount for order more than two oligo at same time
Purification :
Phosphorylation requires additional processing in order to ensure high quality and yield. Purification by PAGE is recommended for long oligonucleotide, extended HPLC is also available.
Quality Controls:
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
| 5' Thiophosphate |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$55.00
$55.00
$110.00
$300.00
$580.00
$55.00
$55.00
$110.00
$300.00
$580.00
|
|
| 3' Thiophosphate |
|
100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$55.00
$55.00
$110.00
$300.00
$580.00
$55.00
$55.00
$110.00
$300.00
$580.00
|
|
Ordering & Contact Information
- Please contact us for additional information or send an email to info@biosyn.com.
- You may also request an online quote.
- To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
- Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
- When using a credit card (
) it will be billed under "Bio-Synthesis, Inc."