Bio-Synthesis offers
photocleavable PC amino C6 oligo modification at various
scales. PC Amino C6 containes a non-nucleosidic moiety that can be used to incorporate
a UV photo-cleavable primary amino group onto the 5’-end of an oligonucleotide.
The amino group is separated from the 5’-end nucleotide base by the photo-cleavable
group and a 6-carbon spacer arm to minimize steric interaction between the amino
group and the oligo
1. The presence of the amino group allows the user
to end label the oligo with a variety of different affinity, reporter or protein,
as well as immobilization on an activated solid support, depending on the application.
The photocleavable group can be selectively cleaved by illumination with UV light
at 300-350 nm spectrum, thereby releasing the marker moiety or detachment of the
oligo from the solid support1. The released oligo contained a 5-phosphate.
PC Amino C6 has been used in peptide-DNA conjugates as photo-cleavable mass marker
(PCMM). The experiment uses a PC DNA oligonucleotide to function as the hybridization
probe and the peptide is then released by UV photo-cleavage during the ionization/desorption
phase of UV-MALDI, and thus serves as a mass tag that identifies the presence of
the target sequence2.
PC Amino C6 is also used to create “caged” oligonucleotides where oligonucleotide
activity is suppressed until released by an external factor (such as UV light).
Caging oligonucleotides would provide new possibilities for controlling biological
mechanisms3.
Contact Bio-Synthesis for Photocleavable Amino C6 Modified
Oligo Service.
Purification:
Photocleavable PC amino C6 modified oligonucleotide can be complished by various
methods. Desalting or cartridge purification is acceptable, however, additional
purificaiton by HPLC is strongly recommended.
Price:
Price/modification. 10%-15% discount for more than two spacer modifications.
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$170.00
$170.00
$195.00
$240.00
inquire
$170.00
$170.00
$195.00
$240.00
inquire
|
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
|
$170.00
$170.00
$195.00
$240.00
inquire
$170.00
$170.00
$195.00
$240.00
inquire
|
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Cleavage Protocol
Optimal cleavage is obtained with exposure to long-wave UV light in the 300-350
nm spectral range. Cleavage releases the oligo with a 5'-phosphate group.
References:
1. Olejnik, J., Krzymanska-Olejnik, E., Rothschild, K.J. Photocleavable aminotag
phosphoramidites for 5’-termini DNA/RNA labeling. Nucleic Acids Res. (1998),
26: 3572-3576.
2. Olejnik, J., Ludemann, H-C., Olejnik, E.K, Berkenkamp, S., Hillenkamp, F., Rothschild,
K.J. Photocleavable peptide-DNA conjugates: synthesis and applications to DNA analysis
using MALDI-MS. Nucleic Acids Res. (1999), 27: 4626-4631
3. Tang, X., Su, M., Yu, LiLi, Lv, C., Wang, J., Li, Z. Photomodulating RNA cleavage
using photolabile circular antisense oligodeoxynucleotides. Nucleic Acids Res.
(2002), 38: 3848-3855.