Photocleavable PC Linker Oligo Modification

Bio-Synthesis offers photocleavable PC Linker Oligo Modification at various scales. This light activatable PC linker can be incorporated at 5', 3' end of an oligonucleotide as well as interally to link two nucleotide sequences, upon photocleavage by UV light, release oligonucleotide yield one 5'-phosphorylated oligo and one 3'-phosphorylated oligo.

The utility of PC Linker for photo-triggered hybridization applications was first demonstrated by Ordoukhanian and Taylor in 1995¹ where they incorporated PC Linker into the sugar-phosphate backbone of a DNA hairpin. Upon irradiation by UV light, photo-cleavage released a 5-phosphorylated 18-mer oligonucleotide having 9X greater hybridization affinity for a complementary DNA strand.

The use of PC Linker has also been explored in designing multi-functional single-stranded nucleotide conjugates for use in in vitro selection of novel DNA or RNA-based catalysts for bio-molecular or organic reactions. PC Linker modified oligonucleotides are the centerpiece of Bruker Daltonik’s genoSNIP, a MALDI-TOF MS based assay system for SNP detection⁴.

Contact Bio-Synthesis for Photocleavable PC Linker Modified Oligo Service.

Purification:

Photocleavable PC Linker modified oligonucleotide can be complished by various methods. Desalting or cartridge purification is acceptable, however, additional purificaiton by HPLC is strongly recommended.

Price:

Price/modification. 10%-15% discount for more than two spacer modifications.

Cleavage Protocol

Optimal cleavage is obtained with exposure to long-wave UV light in the 300-350 nm spectral range. Cleavage releases the oligo with a 5'-phosphate group.

References:

1.Ordoukhanian, P., Taylor, J-S. Design and synthesis of a versatile photocleavable DNA building block. Application to phototriggered hybridization. J. Am. Chem. Soc. (1995), 117: 9570-9571.
2. Hausch, F., Jaschke, A. Multifunctional DNA conjugates for the in vitro selection of new catalysts. Nucl. Acids Res. (2000), 28: e35-00.
3. Hausch, F., Jaschke, A. Multifunctional dinucleotide analogs for the generation of complex RNA conjugates. Tetrahedron (2001), 57: 1261-1268.
4. Wenzel, T., Elssner, T., Fahr, K., Bimmler, J., Richter, S., Thomas, I., Kostrzewa, M. Genosnip: SNP genotyping by MALDI-TOF MS using photocleavable oligonucleotides. Nucleosides Nucleotides Nucleic Acids (2003), 22: 1579-1581.