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Photoswitchable Oligonucleotide Labeling


Bio-Synthesis offers photoswitchable oligo dye labeling. This photoswitchable marker belongs to the photosensitive spiroamide compounds which, upon photoswitching with UV illumination, form transiently fluorescent species. In the dark, the colored and fluorescent form of dye labeled oligo returns to the nonfluorescent state via thermal relaxation. Contact Bio-Synthesis for photoswitchable oligo dye labeling.

Bio-Synthesis photoswitchable labels can be incorporate at any position of an oligonucleotide via post synthetic conjugation, dual HPLC purification is required. Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

Photoswitchable Function Modification Code 5' Prime Internal 3' Prime
Abberior FLIP 565 Abberior FLIP 565 can also undergo two-photon photoactivation with intense IR light (~ 760 nm). Consequently, this label is dedicated for the recording images with optical sectioning, i.e. activating a thin layer of ~ 500 nm. two-photon photoactivation with intense IR light (~ 760 nm). Consequently, this label is dedicated for the recording images with optical sectioning, i.e. activating a thin layer of ~ 500 nm Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

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  • Please contact us for additional information or send an email to info@biosyn.com.
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  • To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."