Rhodamine Dye Oligo Labeling

Rhodamine Dyes

Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labeling techniques for 5' labeling.

This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for internal labeling, a double HPLC purification is required.

Contact your Account Representative for pricing on rhodamine dye labeled oligonucleotides. For additional information, please Contact us.

Price/labeling, purification and synthesis is an additional charge

Dyes	Scales	Label position
		5'-Mod	Internal	3' -mod
Rhodamine GreenTM-X NHS Ester	250 nmol	$195	$220	$195
	1.0 µmol	$250	$300	$250
Rhodamine RedTM-X NHS Ester	250 nmol	$195	$255	$195
	1.0 µmol	$295	$345	$295
TAMRATM	250 nmol	$225	$315	$135
	1.0 µmol	$395	$345	$150
TAMRATM NHS Ester	250 nmol	$125	$200	$135
	1.0 µmol	$195	$250	$195
ROXTM NHS Ester	250 nmol	$170	$200	$160
	1.0 µmol	$225	$275	$225

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo.

Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us.