Enhanced Diagnostic Tools
FEATURES AND BENEFITS
Proteins and peptides purified using Bio-Synthesis's ProteoSpinTM Endotoxin Removal Maxi Kit can be used directy in numerous downstream applications with no additional clean-up or purification required. Downstream applications include:
Figure 1. Endotoxin-Free Proteins. Endotoxins were removed from 1 mg protein samples in triplicate using the ProteoSpin™ Endotoxin Removal Maxi Kit. Samples containing 1 mg of BSA were spiked with endotoxins (2.3 EU/mg). The endotoxin-spiked BSA was then cleaned, in triplicate, using the ProteoSpinTM Endotoxin Removal Maxi Kit by using 50 µL of the provided Endotoxin Removal Solution in the presence of Isopropanol. On average, the endotoxin levels in the samples were reduced to 0.07 EU/mg of protein.
Figure 2. High Protein Recovery. An average protein recovery of greater than 95% was achieved when endotoxins were removed from 0.5 mg of protein in triplicate. There is very little loss of protein associated with using the ProteoSpin™ Endotoxin Removal Maxi Kit.