Spiked Oligos
Spiked bases refer to non-equimolar mixtures of bases. Spiked oligonucleotides are
useful when a determined ratio of degenerated bases is required at precise positions
within the sequence. This is generally required when one wants to use the preferential
genetic codons rather than to degenerate the wobble position with a statistical
mix of dNTP.
Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Quality Controls
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.