Enhanced Diagnostic Tools
Protein A, Protein G, Protein A/G and Protein L are native and microbial recombinant proteins which have binding sites for the Fc portion of mammalian IgGs. The capacity of these proteins for IgG varies with the species. The interaction between the various proteins and IgG is not equivalent for all species or all antibody subclasses. In general, IgGs have a higher affinity for Protein G than for Protein A, and Protein G can bind IgG from a wider variety of species. The affinity of various IgG subclasses, especially from mouse and human, for Protein A varies more than for Protein G. Protein A can, therefore, be used to prepare isotypically pure IgG from some species. Protein L has an affinity for kappa light chains from various species. It can be used to purify monoclonal or polyclonal IgG, IgA, and IgM as well as Fab, F(ab1)2, and recombinant scFv fragments that contain kappa light chains.
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