Enhanced Diagnostic Tools
When membranes of blotted proteins are probed with a secondary gold conjugate, the presence of a target protein is indicated with a red color upon binding of the gold conjugate.
When combined with silver enhancement (i.e. deposition of silver onto bound gold colloid turning the spot dark in color) sensitivity in western blot and dot-blot applications rivals that of colorimetric detection methods. In addition, secondary gold probes adapt well to standard western blot protocols and little changes are necessary to your current detection scheme.
Figure 1. Example dot-blot assay for streptavidin gold conjugate (top left) and streptavidin silver conjugate (top right) before and after enhancement using silver enhancement kit for membranes. Bottom picture illustrates and highlights the difference in appearance (color) of 50nm anti-human IgG noble metal nanoparticle conjugates prepared using NHS-activated gold nanoparticles, NHS-activated gold nanourchins and NHS-activated silver nanoparticles, respectively.
1. M. Moeremans, et al., Journal of Immunological Methods, 1984, 74, 353
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