Enhanced Diagnostic Tools
Peptide libraries allow for the search of peptide based bioactive molecules by screening large numbers of peptides for their activity. Often, this is not possible with the help of traditional chemical approaches. Therefore, combinatorial methods have been designed over the years using peptide chemistries. Synthetic peptide libraries can be prepared as either mixtures or sets of individual peptides. In the past both, tert-butyloxycarbonyl (tBoc) or 9-flurenylmethyloxycarbonyl (Fmoc) solid phase peptide synthesis methods (SPPS) have been used for the synthesis of large numbers of individual peptides. However, in recent years the automated Fmoc-based SPPS method appears to have become the dominant synthesis method. The availability of commercial automated SPPS instruments and methods makes the development of assays and screening strategies now much easier.
Combinatorial chemistry is a branch of applied chemistry using rapid synthesis and screening methods and strategies to screen large mixtures of individual but related molecules for their biological activities. Combinatorial chemistry in drug discovery represents a merging of chemistry and biology that involves the use of chemical synthetic methods coupled with biological screening assays. The resulting compound libraries can be mixtures or sets of individual molecules or chemical structures such as peptides of defined length. However, combinatorial chemistry is not only useful for the synthesis of peptide libraries but also for a whole host of other molecular libraries such as oligonucleotides, either made up of natural or modified nucleotide monomers. In pharmaceutical research combinatorial chemistry is used for the development of biologically active compounds such as new drugs and catalysts, some of which can be peptide-based.
Peptide libraries have now become key research tools for the screening of bioactive compounds by using large numbers, tens of thousands or even billions, of peptides. The synthesis of peptide libraries is not practically attainable with the help of traditional chemical approaches.
The use of libraries containing large collections of peptides that can range from hundreds to millions or even to billions of different sequences allows for high-throughput screening of biological functions using monoclonal and polyclonal antibodies, receptors, enzymes or other target molecules. The chemical diversity of amino acids used coupled with the large number of sequences in a library results in the power of this technology. For drug screening and development, peptide libraries are useful for identifying of ligands that can serve as leads for pharmaceutical development or other purposes.
Bio-Synthesis has implemented Peptide Library Tools in its rapid high-throughput parallel peptide synthesis platform. These libraries can be used to screen highly active compounds such as antigenic peptides, receptor ligands, antimicrobial compounds and enzyme inhibitors.
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