Purification and Quality Control of Long RNA Synthesis by Transcription

After long RNA synthesis by transcribing from DNA template, the unmodified RNA transcripts from standard DNA synthesis are purified by phenol-chloroform extraction and ethanol precipitation following with spin column chromatography.  For capped RNA synthesis, non-radioactively labeled RNA or highly specific activity radiolabeled RNA probes, spin column chromatography is the preferred method. When using high purity RNA transcripts for labeled RNA probes for RNase protection assay or foot printing experiments, we recommend gel purificaiton of the transcription product.

Bio-Synthesis's Long RNA Synthesis transcription service includes phenol-chloroform extraction and ethanol precipitation. Final products are further purified using spin column chromatography. Gel purification is available for an additional fee.

After transcription from template DNA, RNA transcripts concentration are determined by measuring with ultraviolet light absorbance at 260 nm wavelength. Evaluation of transcript length, integrity and quantity are further analyzed on an appropriate agarose gel or polyacrylamide gel.