Enhanced Diagnostic Tools
TEAB a Versatile, Volatile Buffer for Biological Applications
In the biochemical lab, there is the need to use a variety of buffers for chemical/biological reactions and for purification purposes. One of the problems is buffer incompatibility, therefore, in many situations it is very desirable to have a solvent that does not interfere with subsequent steps. A volatile solvent is ideal. One of the few known volatile solvents is Triethyl Ammonium Bicarbonate (TEAB)
Preparation of 1 M TEAB buffer:
Removal of TEAB buffer: The buffer can easily and conveniently be removed by simple lyophilization or by using a speed vac.
Oligonucleotide Isolation: One useful applications of TEAB is for eluting crushed PAGE gel from a DE-52 bed; generally 3x1 ml aliquots are applied to extract the oligo bound to the DE-52 bed. The TEAB is evaporated off by speed vac, and then the oligo can be quantitated by uv spectrometry.
Bio-Synthesis, Inc. has been producing synthetic oligonucleotides for over 25 years. BSI also specializes in RNA, other modified oligos, as well as providing cross-linking of various types of biomolecule using our optimized bioconjugation strategies. There are a number of applications on gene expression inhibition and related anti-sense studies. Synthetic peptides and peptide antibodies are used for a number of biological relevant applications, in the areas of proteomics, epigenetics, immune regulation, post translational modifications, antisense, gene expression control, RNA interference and more. For more product information, visit: www.biosyn.com.
If you have a query regarding any of our products or services,
please don't hesitate to submit your question below. A member of our support team
will review and answer your question as quickly as possible.