TEAB a Versatile, Volatile Buffer for Biological Applications
In the biochemical lab, there is the need to use a variety of buffers for chemical/biological reactions and for purification purposes. One of the problems is buffer incompatibility, therefore, in many situations it is very desirable to have a solvent that does not interfere with subsequent steps. A volatile solvent is ideal. One of the few known volatile solvents is Triethyl Ammonium Bicarbonate (TEAB)
Preparation of 1 M TEAB buffer:
- Fill a 2 liter Erlenmeyer flask, with 3-4 pounds of crushed dry ice (solid carbon dioxide), cover the flask and connect a tygon tubing from the side arm
- In a separate 2 liter flask, place a 1 M aqueous solution of triethylamine
- Place the end of the tygon tubing into the bottom of the flask containing the aqueous TEA, and observe the bubbling of gaseous Co2 into it
- Bubble CO2 for about 3-4 hours or until the pH of the aqueous TEA reaches pH 8.5 5. Store in a screw cap bottle and keep at 4 degrees Celsius
Removal of TEAB buffer: The buffer can easily and conveniently be removed by simple lyophilization or by using a speed vac.
Oligonucleotide Isolation: One useful applications of TEAB is for eluting crushed PAGE gel from a DE-52 bed; generally 3x1 ml aliquots are applied to extract the oligo bound to the DE-52 bed. The TEAB is evaporated off by speed vac, and then the oligo can be quantitated by uv spectrometry.
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