Enhanced Diagnostic Tools
Thiol (SH) group can be linked to the 5' or 3' terminus of an oligonucleotide. This
modification is used for oligo activation prior to linking to various types of molecules
or support. The thiol groups can be used to attach a variety of products to the
oligonucleotide. Common thiol-reactive groups include maleimides, halogens, iodoacetamides
and pyridyldisulfides. This DNA modification is commonly used for the attachment
of enzymes such as horseradish peroxidase or alkaline phosphatase.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.
Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.