Enhanced Diagnostic Tools
A universal base analogue have ability to replace any of the four natural bases without significantly effecting either melting behavior of dupelxes or disrupting normal activities of modified oligonucleotide. These Universal bases can be used to replace degenerate bases, and have the advantage that the hybridization probe is not diluted by the non-pairing components of the degeneracy.
A number of such analogues are available at Bio-Synthesis. Universal nucleobases we offered includes: 3-nitropyrrole 2’-deoxynucleoside, 5-nitroindole, P and K nucleoside analogues that are able to hybridise efficiently with respectively both purine (A-G) or pyrimidine (C-T). It is usually not recommended to introduce universal bases in template strands (e.g. PCR primers), as DNA polymerases may dissociate from the template when encoutering these bases. These universal bases can be incorporate at 5', 3' or internal position of an oligonucleotide.
Contact Bio-Synthesis for universal bases..
All oligonucleotide synthesized at Bio-Synthesis is quality check by using either MALDI-TOF mass spectrometry, analytical HPLC,or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform custom formulation and quality control tailored to meet your specific requirements.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.