Enhanced Diagnostic Tools
Please fill out this form and send it in along with your sample
Total amount of protein if providing protein in gel slice :
Please run the gel as you normally would and can stain with something like
Coomasie without any harmful effects and make the final step a distilled water
rinse to remove any excess acid or alcohol which was used in the preparation.
Then put the gel slice(s) in a tube without buffer (just the gel pieces by
themselves) and provide us with the amount of protein you believe is represented
in the gel so we can aliquot it properly for each injection. The target amount
is 3-4 mg of protein (from gel) and another 0.5 mg for ELISA (soluble protein in
PBS buffer) as only ~60% of protein loaded will be present.
Bio-Synthesis Inc., in compliance with federal regulations, presents each animal
use project to IACUC (Institutional animal Care and use committee) for approval
prior to project initiation. Criteria for acceptance of a project are
established in 9CRF. chapter 1: Animal and Plant Health Inspection Service,
USDA, subchapter A-Animal Welfare.
To Expedite initiation of your project, please complete the following statement
I certify that the supplied antigen is non-toxic, non-pathogenic,
non-carcinogenic and does not present specific hazard to the animal and it has
no known safety concerns to lab personnel handling this antigen. I also certify
that the supplied antigen is not derived from human material. Animal/species
selection or number required for this project are appropriate and do not
unnecessarily duplicate previous work. This certification is required for
inclusion in our IACUC approval program.
BSI will NOT accept protocols that do not properly identify each antigen and its
source and do not conform to these IACUC guidelines for handling and use of
animals for research. We will not start your project without this certificate.
All Projects are performed on best effort basis. No specific assurance,
expressed or implied, is given that animals will produce a desired immune
response. We guarantee an acceptable, minimal (1:1K) ELISA titer or the project
is repeated at no cost.
I have read and agree to the above certification*