Oligo Designer
Sequence
5'-
Please review instructions below for entering your sequence and modifications
-3'
OD / µg / nmol
OD
µg
nmol
OD/µg Required
Must be greater than 0
Results
Sequence:
nmoles:
µg:
OD
260
:
nmole/OD
260
:
µg/OD
260
:
Anhydrous MW:
Tm (Salt Adjusted):
GC Content:
Extinction Coefficient:
Length:
How to enter your sequence:
DNA bases: A, G, C, T
RNA bases: rA, rG, rC, rU
2'-O-methyl RNA bases: mA, mG, mC, mU
2' Fluoro RNA bases: fA, fG, fC, fU
Bridged nucleotides (+): +A, +G, +C, +T
Phosphorothioate bond: *
How to insert modifications:
Enter your sequence without modifications
Place the mouse cursor at a point in the sequence where you would like to insert a modification
You will see a flashing cursor at that location "|"
Move the mouse pointer to the modifications table and click on the link for the modification you wish to insert. (these are the blue links found on the right side of the calculator)
The desired modification will be inserted automatically at the cursor location in the sequence