Oligo Designer

Sequence
5'- -3'
OD / µg / nmol
Results
Sequence:
nmoles:
µg:
OD260:
nmole/OD260:
µg/OD260:
Anhydrous MW:
Tm (Salt Adjusted):
GC Content:
Extinction Coefficient:
Length:
(invabasic)s InvAbs
[L1-BMPS-eGLP1] L1-BMPS-eGLP1
[L1s-BMPS-eGLP1] L1s-BMPS-eGLP1
1,3-DIAZA-2-OXOPHENOXAZINE tCo
1-Methyl-A N1-Me-rA
1-Methyl-PseudoUridine 1-Me-Psd U
2' Methoxy Inosine mI
2',2'-difluoro cytidine dfC
2,6-diamino purine rDAP
2'-Amino C NH2-C
2'-Amino U NH2-U
2-Cystamine dG 2-Cystm-dG
2'-dU dU
2'-F rU thiophosphoramidite fUss
2'-F T fT
2'-F-5-Me-U fT
2'-F-5-Me-U-ANA FANA-T
2'-F-Arabinonucleic Acid (2'-F-A-ANA) FANA-A
2'-F-Arabinonucleic Acid (2'-F-Ac-C-ANA) FANA-C
2'-F-Arabinonucleic Acid (2'-F-G-ANA) FANA-G
2'-F-Arabinonucleic Acid (2'-F-U-ANA) FANA-U
2-Fluoro-deoxyInosine 2-F-dI
2'-Methoxy T mT
2'-OMe-5-Me-U mT
2'-OMe-Ac-C-PACE mC-PACE
2'-OMe-A-PACE mA-PACE
2'-OMe-G-PACE mG-PACE
2'-OMe-rU thiophosphoramidite mUss
2'-OMe-U-PACE mU-PACE
2'-Propargyl rA Prg A
2'-Propargyl rC Prg C
2'-Propargyl rG Prg G
2'-Propargyloxy U 2'-Propar U
2'-TBDMS rU thiophosphoramidite rUss
3' Azide PEG4 N3-PEG4
3' Dabcyl CPG 3-Dabcyl
3' Hexynyl 3-Hexynyl
3'-(6-FAM) CPG 3-6FAM
3'-(6-Fluorescein) CPG 3-6Flurecin
3'-6-Fluorescein CPG 3-6Fluorescein
3'-6-Fluorescein Serinol CPG 3-6-Fluorescein Serinol
3'-Biotin CPG 3-Biotin
3'-Black Berry Quencher 650 II 3-BBQ 650 II
3-Cyanovinylcarbazole Phosphoramidite (CNVK) CNVK
3'-ddC ddC
3-deaza-3-Me dA 3-deaza-3-Me dA
3'-Fluorescein CPG 3-Fluorescein
3'-Propargyl-5-Me-dC 3'-Prop-5-Me dC
3'-symmetric doubler sym-dbler-chemgen
3'-symmetric doubler-Glenres Sym-dbler-Glen
3'-TAMRA CPG 3'-TMR
3'-TAMRA-HG 3'-TMR-HG
3'-Thio-dI 3-Thio-dI
4-chloro-3-trifluoromethylphenyl isocyanate 4-Cl-3-TFMP-IsoCyan
4-Thio dT 4-Thio-dT
4-Thio-dU 4-Thio-dU
4-ThioUridine 4-Thio U
5' Adenylation 5rApp
5' BHQ-1 5BHQ-1
5' BHQ-2 5BHQ-2
5' Biotin 5 Biotin
5' C6 Cholesteryl C6 Chlst
5'-Amino TEG Amino TEG
5'-Azido-dT 5-N3-dT
5-Br dC 5Br-dC
5-Br-dU 5-Br-dU
5-carboxy-dC 5-carboxy-dC
5'-CDPI3 MGB™ Phosphoramidite 5'-MGB
5'-Cholesterol TEG/ no DMT 5-Cholest TEG
5'-Dabcyl Phosphoramidite 5-Dabcyl
5'-Dichloro-dimethoxy-Fluorescein Phosphoramidite II JOE-TM
5-Ethynyl-dU Ethynyl dU
5-Ethynyl-U Ethynyl U
5-F dC 5-F-dC
5-F-dU CEP 5-F-dU
5'-Fluorescein Phosphoramidite 6-FAM
5-Formyl-dC Formyl-dC
5-Formylindole Aldehyde
5'-Hexachloro-Fluorescein Phosphoramidite HEX
5-Hydroxymethyl dC 5-Hydroxymethyl dC
5-I dU 5-I dU
5-I-dC 5-I-dC
5'-Iodide dT 5-I-dT
5-Me 2'-OMe Cytidine 5-Me-mC
5-Me dC brancher 5-Me dC Brch
5-Me isodC 5-Me isodC
5-Me-2'-F C fCm
5-Me-2'-MeO rC 5-Me-Cm
5-Me-C-TOM-CE 5-Me rC
5-Me-dC 5-Me dC
5-Me-dC Brancher 5-Me-dC Brancher
5-MethylUridine 5-Me-U
5'-NED NED
5-Nitroindole Nitroindole
5-Octadiynyl-dU 5-OCt-dU
5-OH-dU 5-Hydroxy dU
5'-OMe dT 5-OMe dT
5'-Palmitate-C6-CE Phosphoramidite palmitoy-AmC6
5-Propargyloxy dU 5-Propar dU
5'-Pyrene Cap Py Cap
5-ROX C2 Maleimide 5-ROX-M
5-ROX NHS ester 5-5ROX
5-taurinomethyl-2-thio rU 5-TauMe-2-S rU
5'-Tetrachloro-Fluorescein Phosphoramidite TET-TM
5'-VIC VIC
6-FAM NHS 6-FAM-N
6-Fluorescein Phosphoramidite IntFAM
6-Fluorescein Serinol Phosphoramidite SerinolFAM
6-HEX NHS 6-HEX N
6-JOE amidite 6-JOE
6-ROX C2 Maleimide 6-ROX-M
6-ROX NHS ester 5-6ROX
6-TAMRA-by 6-TAMRA-by
6-Thio-G 6-Thio-G
6-Thiol-dG 6-Thiol-dG
7-amido-7-deaza-2'-deoxyguanosine 7-Amid-7-deA-dG
7-deaza- dG 7-deaza- dG
7-deaza-7-Me dG 7-deaza-7-Me dG
7-Deaza-8-aza-dG PPG
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester Methoxycoumarin
8-Azanebularine 8-Aza NB
8-Oxo-dG 8-Oxo-dG
8-Oxo-rG 8-Oxo-rG
A366 A366
Abasic Ab
Abasic II Abasic II
Abberior Cage 500 NHS Abb Cage 500N
Abberior Cage 532 NHS Abb Cage 532N
Abberior Cage 552 NHS Abb Cage 552N
Abberior STAR 520SXP, NHS ester Abberior520SXP-N
Abberior STAR 635 NHS Abberior STAR 635
Abberior® STAR 580 Abb 580
Acridine Acridine
Acrydite TM (Methacrylate C6) Acrydite
AF 350 MAL AF 350M
AF 532 NHS AF532
AFDye 594 NHS AFD 594
Aldehyde C2 Aldehyde C2
Alexa Fluor 350 NHS AF 350
Alexa Fluor 405 NHS AF405
Alexa Fluor 488 C5 Maleimide AF 488M
Alexa Fluor 488 NHS AF 488
Alexa Fluor 532 NHS AF 532
Alexa Fluor 546 NHS AF 546
Alexa Fluor 555 NHS AF555
Alexa Fluor 568 NHS AF 568
Alexa Fluor 594 NHS AF594
Alexa Fluor 610-X NHS AF 610-X
Alexa Fluor 633 NHS AF 633N
Alexa Fluor 647 AF 647
Alexa Fluor 647 Maleimide AF 647M
Alexa Fluor 660 NHS AF 660
Alexa Fluor 680 NHS AF 680
Alexa Fluor 700 NHS AF 700
Alexa Fluor 750 C5 Maleimide AF 750M
Alexa Fluor 790 NHS AF 790
Alexa Fluore 514 AF 514
Alexa Fluore 750 AF 750
Alexa Fluor™ 430 NHS Ester Copy AF 430N
Alkyne CPG 3-Alkyne
Alkyne Serinol Alkyne
Alkyne-PEG5 NHS Alkyne-PEG5
Am-But-carboxyl Am-But-carboxyl
Amino 7 modifier Amino 7
Amino C6 AmC6-Glen
Amino C6 dG AmC6 dG
Amino C6-Chemgen AmC6-Chemg
Amino linker-amc Amino linker-amc
Amino Modifier C12 AmC12
Amino-Modifier 5 Am5
Amino-Modifier C2 dT AMC2 dT
Amino-Modifier C3/ Chemgene CPG AmC3-Chem
Amino-Modifier C3/ Glen Am C3
Amino-Modifier C6 dA AmC6 dA
Amino-Modifier C6 dC AmC6 dC
Amino-modifier C6 dT AmC6 dT
Amino-Modifier C6-U AmC6 U
Amino-Modifier C7 AmC7
Amino-Modifier Serinol Amserinol
Amino-Modifier Serinol Phosphoramidite Serinol Amino
Amino-Modifier TEG AmTEG
Aminooxy-Modifier-11-CE Phosphoramidite Amox11
ATTO 390 NHS ATTO 390
ATTO 425 NHS ATTO 425
ATTO 430LS NHS ATTO430LS
ATTO 465 NHS ATTO 465
ATTO 488 NHS ATTO 488
ATTO 490LS NHS ATTO 490LS
ATTO 495 NHS ATTO 495
ATTO 514 NHS ATTO 514
ATTO 520 NHS ATTO 520
ATTO 532 NHS ATTO 532
ATTO 550 NHS ATTO 550
ATTO 565 NHS ATTO 565
ATTO 575Q NHS ATTO 575QN
ATTO 590 NHS ATTO 590
ATTO 594 NHS ATTO 594
ATTO 610 NHS ATTO 610
ATTO 633 NHS ATTO 633
ATTO 643 NHS ATTO 643N
ATTO 647N NHS ATTO 647N
ATTO 655 NHS ATTO 655
ATTO 665 NHS ATTO 665
ATTO 700 NHS ATTO 700 NHS
ATTO 725 NHS ATTO725N
ATTO 740 NHS ATTO 740
ATTO Methlyene Blue2 maleimide ATTO MB2 maleimide
ATTO Methlyene Blue2 NHS ester ATTO MB2 NHS ester
ATTO Rho 101 NHS ATTO Rho 101
ATTO Rho 6G NHS ATTO-Rho-6G
ATTORHO 11 NHS ATTORHO 11
Azide C6 NHS Azide C6
Azide CPG-By Azide-By
Azide PEG4 NHS Azide PEG4
Azido butyrate NHS C4 Azide
Azido PEG NHS Azido PEG
Azido PEG24 NHS Azide PEG24
Azido PEG4 NHS Azido PEG4
Azidobutyrate NHS Azidobutyrate
Azido-PEG3-Maleimide Azd-PEG3-Mal
Azido-PEG3-N Azido-PEG3-N
Azobenzene AzBen
BBQ 650 NHS BBQ 650N
BBQ-650 CPG 3-BBQ-650
BCN PEG4 NHS BCN-PEG4N
BG-LGA Snap-tag BG-LGA
BHQ-1 CPG BHQ-1
BHQ-1 dT BHQ-1 dT
BHQ-1 NHS ester BHQ-1 NHS
BHQ-2 CPG BHQ-2
BHQ-2 DMT int-BHQ-2
BHQ-2 dT BHQ-2 dT
BHQ-3 CPG BHQ-3
BHQ-3 NHS ester BHQ-3N
Biotin dT Biotin dT
Biotin Phosphoramidite IntBiotin
Biotin-desthiol TEG DesthioBiotin TEG
Biotin-PEG3-NHS Biotin PEG3-NHS
Biotin-PEG4-NHS Biotin-PEG4
BiotinTEG BiotinTEG
Bodipy581-591 NHS Bodipy581-591
BODIPY™ 493-503 NHS BODIPY 493-503N
Bromohexyl Bromohexyl
Butyric acid BA
C3 Spacer SpC3
C4 DBCO NHS C4-DBCO
C4 Spacer SpC4
C6 hydrzide C6NHNH2
C8-Alkyne-dC C8-Alkyne-dC
C8-Alkyne-dC-CE C8Alkyne dC
C8-Alkyne-dT C8Alkyne dT
C8-TIPS-Alkyne-dC C8TIPS Alky dC
C8-TMS-Alkyne-dC C8TMS Alky dC
C8-TMS-Alkyne-dT C8TMS Alky dT
CAL Fluor Red 610 Amidite CAL Red 610
Carboxy Carboxy
Carboxy-dT Carboxy dT
Carboxy-Modifier C10 C10NHS-Acid
Carboxy-Modifier C5 C5acid
Cascade Blue Acetyl Azide Cascade Blue
CDPI3 MGB™ CPG MGB
cEt-A kA
cEt-C kC
cEt-G kG
cEt-U kU
CF® Dye 430 Succinimidyl Ester CF430N
CF® Dye 750Succinimidyl Ester CF750N
CF405L NHS CF405L
CF405S NHS CF405S
CF800 NHS CF 800
Cholesterol CPG 3-Chl-GP
Cholesteryl-TEG 3CPG/5DMT Cholesteryl-TEG
Chromeo 642 NHS Chromeo 642
Circularization Circ
Cis-syn Thymine Dimer Cis-syn T
Cy3 NHS-GE Cy3-GE
Cy3 NHS-LP Cy3-LP
Cy3B Cy3B
Cy3B Maleimide Cy3B-M
Cyan 500 NHS Cyan 500
Cyanine 3 Cy3
Cyanine 3 CPG 3-Cy3
Cyanine 5 amidite Cy5
Cyanine 5 Maleimide Cy5-M
Cyanine 5 NHS Cy5-NHS
Cyanine 5.5 Cy5.5
Cyanine 5.5 NHS Cy5.5 NHS
Cyanine 7 NHS Cy7N
Cyanine 7.5 NHS Cy7.5
Cyanine2 NHS ester Cy2N
Cyanine5 CPG 3-Cy5
dA Methyl phosphoramidite dA-POMe
Dabcyl NHS Dabcyl-N
Dabcyl-dT Dabcyl-dT
Dansyl NHS ester Dansyl N
dA-P-Methyl dA-PMe
DBCO PEG12 NHS DBCO-PEG12N
DBCO PEG4 NHS DBCO-PEG4N
DBCO PEG5 NHS DBCO PEG5
DBCO-dT DBCO-dT
DBCO-PEG1-CH2CH2COON DBCO-PEG1-CH2CH2COON
DBCO-TEG DBCO-TEG
dC methyl Phosphoramidite dC-POMe
dC-p-Methyl dC-PMe
dCss dCss
ddA ddA
ddT ddT
Deep Dark Quencher-I DDQ-I
Deoxy Inosine dI
dG Methyl phosphoramidite dG-POMe
dG-P-Methyl dG-PMe
dGss dGss
DHA-AMC7 CPG DHA-Am7
Dig-NHS Digoxigen
Dihydro dU DH-dU
DihydroUridine Dihyd-U
Diphosphate PP
disuccinimidyl suberate DSS-NHS
Dithiol Serinol Phosphoramidite (DTPA) Dithiol Serinol
DMT-ON DMT-ON
DNP-TEG DNP-TEG
dP dP
dSpacer dSp
dT Methyl phosphoramidite dT-POMe
dT-P-Methyl dT-PMe
dT-thiophosphoramidite dTss
DTX-01-09 DTX-01-09
DTX-01-10 DTX-01-10
DY 415 NHS DY 415
Dy 634 Dy 634
DY 652 NHS DY 652
DY 754 NHS DY 754
DY-351XL-NHS-ester DY-351XL-N
DY-395XL NHS DY-395XL
DY-485XL NHS DY-485XL
DY-510XL NHS DY-510XL
DY-511XL NHS DY-511XL
DY-520XL NHS DY-520XL
DY-521XL NHS DY-521XL
DY560 NHS DY560
DY682 DY682
DY750 NHS DY750
DY751 NHS DY751
DyLight 350 Maleimide DyLight 350M
DyLight 350 NHS DyLight 350N
DyLight 405 NHS Ester DyLight 405N
DyLight 485-LS NHS DyLight 485-LS
DyLight 488 NHS DyLight 488
DyLight 510-LS NHS DyLight 510-LS
DyLight 515-LS NHS DyLight 515-LS
DyLight 550 NHS DyLight 550
Dylight 650 Dylight650
DyLight 755 NHS DyLight 755
DyLight 800 NHS DyLight 800
DYQ-3 NHS DYQ-3
DYQ-4 DYQ-4
DYQ-425 NHS DYQ-425N
DYQ-700 NHS ester DYQ-700
Eclipse Quencher Eclipse
EDTA-C2-dT EDTA-C2-dT
EQ-1 NHS EQ-1
Etheno dA Etheno dA
fA fA
fAs fAs
fC fC
fCs fCs
Ferrocene C6 amidite Ferrocene C6
Ferrocene CPG 3- Ferrocene
Ferrocene NHS Ferr-NHS
Ferrocene-dT Ferrocene-dT
fG fG
fGs fGs
fluorescein isothiocyanate FITC
Fluorescein Phosphoramidite IntFluor-S
Fluorescein-dT Phosphoramidite IntFluor-dT
FormylBenzamide Formy-Benz
fU fU
fUs fUs
G capping Gppp
GalNAc C3 CPG GalNAc C3-b
GalNAc C3 Phosphoramidite GalNAc C3
Glucose Glucose
Glycine Gly
Hexane diol SpC6
HEX-dT HEX-dT
Hexynyl Phosphoramidite hexynyl
HiLF750 NHS HiLF750
Hydroxymethyl dU HOMe-dU
Inosine rI
Int Uni-LinkTM Amino Modifier iUniAmM
Internal BHQ-1 iBHQ-1
Internal BHQ-3 Int BHQ-3
invabasic InvAb
Inverted dA Ivt dA
Inverted dSpacer Inv dSp
Inverted dT idT
Inverted mA Ivt mA
Inverted mC Ivt mC
Inverted mG Ivt mG
Inverted mU Ivt mU
Inverted rA Ivt rA
IRDye 680RD NHS IRDye 680RD
IRDye 700 amidite IRD 700
IRdye 700DX NHS IRDye 700
IRDye QC-1 IRDye QC-1
IRDye800 CW IRDye800 CW
IRDye800 CW-Mal IRDye800 CW-Mal
isodC isodC
isodG isodG
Janelia 525 NHS Janelia 525
Janelia Fluor 585, SE(NHS ester) JF585N
Janelia Fluor® 646, SE JF 646N
JOE NHS JOE
KSHLDKH AS
L-dA L-A
L-dC L-C
L-dG L-G
L-dT L-T
LightCycler Red 610 NHS LC Red610
LightCycler Red 640 NHS LC Red640
Lignoceric acid Lignoceric acid
LNA-A AL
LNA-G GL
LNA-mC CL
LNA-T TL
L-RNA A L-rA
L-RNA C L-rC
L-RNA G L-rG
L-RNA U L-rU
mA mA
Mal-NH-PEG2-BCN BCN-PEG2-Mal
Mal-PEG24-amido-PEG24-NHS Mal-PEG24-amido-PEG24-NHS
mAs mAs
mC mC
mCs mCs
Methionine Methionine
Methyl-dPEG4 Me-PEG4
methylene blue NHS-Bios MB-Bios
methyltetrazine NHS Methyltetrazine
Methyltetrazine PEG4-CC Me-Tetrazine
Methyltetrazine-PEG5-BP Methyltetrazine-PEG5
mG mG
MGB EQ MGB EQ
mGs mGs
MOE-5MeC eCm
MOE-A eA
MOE-C eC
MOE-G eG
MOE-T eT
MOE-U eU
mU mU
mUs mUs
N2-Amino-Modifier C6 dG AmC6 dG
N2-Methyl dG N2-Me dG
N3-Methyl thymidine N3-Me T
N6,N6-Dimethyl 2'-OMe Adenosine DMe-mA
N6,N6-Dimethyl Adenosine DMe-rA
N6-Me-2'-OMe A M6mA
N6-Me-2'-OMe A Sul M6mAs
N6-Me-dA N6-Me-dA
N6-Methyl-A N6-Me-rA
N7-Me-G capping M7Gppp
N-AcetylCysteine N-AcCystein
Nap-dU Nap-dU
Nonane Diol Sp C9
NPOM Caged dT NPOM-dT
NTA maleimide NTA
O6-Me-dG O6-Me-dG
Oregon Green 488 NHS OG 488
Oregon Green 514 NHS OG 514
Pacific Blue maleimide Pacific Blue M
Pacific Blue NHS Pacific Blue
Pacific Green NHS Pacific Green
Pacific Orange NHS Pacific Orange
PAL-C PAL-C
Palmitic acid Palmitic acid
PC Amino-Modifier PCAm
PC azide NHS PC azide
PC Biotin PC Biotin
PC Biotin NHS PC-Biotin-NHS
PC Linker PC-Linker
PC Spacer SpPC
pdC pdC
pdU pdU
PEG 20K NHS PEG20KN
pHAb NHS pHAb
Phosphate Phos
phosphorothioate Phosthi
Piperidine PPRD
Polyethyleneglycol 2000 CED amidite PEG2000
PromoFluor 840 NHS PromoFl 840N
Protected Biotin Amidite Pr-Biotin
PseudoUridine Psd-rU
Psoralen C6 Psoralen C6
Puromycin CPG 3-Puromycin
QSY 21 NHS QSY 21
QSY 7 NHS QSY 7
QSY 9 NHS QSY 9
Quasar 570 Quasar570
Quasar 570 CPG 3- Quasar 570
Quasar 570 NHS Quasar 570N
Quasar 570-GP Quasar 570-GP
Quasar 670 Quas 670
Quasar 670 NHS Quas 670N
Quasar 670-GP Quasar 670-GP
Quasar 705 Amidite Quasar 705
rA rA
rAs rAs
rC rC
rCs rCs
rG rG
rGs rGs
Rhodamine Green NHS Rhodamine Green
ROX C2 Maleimide ROX-M
rR{rA+rG} rR
rU rU
rUs rUs
rW{rA+rU} rW
rY{rC+rU} rY
SeTau-665 NHS SeTau-665
SeTau-670 NHS SeTau-670
SeTau-680 NHS SeTau-680
SIMA Phosphoramidite SIMA
SMCC-Mal Maleimide
Spacer 18 Sp18
Spacer 9 Sp9
Spacer C12 SpC12
SPDP SPDP
Spermine Z
Square-660 NHS Square-660
STAR 580 NHS ester STAR 580
STAR 635P NHS ester STAR 635P
Sulf-Cy5 NHS S-Cy5-NHS
SynBase Cholesterol CPG 3-Chl-SB
Tacrolimus NHS Tacrolimus
TAMRA TMR
TAMRA-dT TAMRA-dT
TAMRA-NHS TMR-NHS
tCnitro tCnitro
TCO NHS TCO N
TCO PEG12 NHS TCO PEG12
TCO PEG4 NHS TCO-PEG4 N
temp temp
TetraEthyloxy Glycol(TEG) Spacer Sp12
Tetrazine NHS Tetrazin N
Tetrazine PEG5 NHS Tetrazine PEG5
Texas Red Texas Red
Thiol C3 Thiol C3 SH
Thiol C3 disulfide Thiol C3 S-S
Thiol modifier C6 dT Thio-C6-dT
Thiol Modifier C6 SH Thiol C6 SH
Thiol Modifier C6 S-S Thiol C6 S-S
Thymidine Glycol dT-Glycol
Tocopherol-TEG Tocopherol-TEG
Trebler Long Trebler
Trebler short s-trebler
Tri-GalNAc CPG-By GalNAc-Khvo
triphosphate PPP
Trp-dU Trp-dU
Uaq Cap Uaq Cap
UNA-A UNA-A
UNA-C UNA-C
UNA-G UNA-G
UNA-U UNA-U
Val-Cit-PABA-mC Val-Cit-PABA-mC
VIC 3'-CPG 3'-VIC
VIC NHS VIC-N
Yakima Yellow Phosphoramidite Yakima Yellow

How to enter your sequence:
  • DNA bases: A, G, C, T
  • RNA bases: rA, rG, rC, rU
  • 2'-O-methyl RNA bases: mA, mG, mC, mU
  • 2' Fluoro RNA bases: fA, fG, fC, fU
  • Bridged nucleotides (+): +A, +G, +C, +T
  • Phosphorothioate bond: *
How to insert modifications:
  • Enter your sequence without modifications
  • Place the mouse cursor at a point in the sequence where you would like to insert a modification
  • You will see a flashing cursor at that location "|"
  • Move the mouse pointer to the modifications table and click on the link for the modification you wish to insert. (these are the blue links found on the right side of the calculator)
  • The desired modification will be inserted automatically at the cursor location in the sequence