The need to develop an efficient and precise detection technique for multiple, low-abundance protein has played an important role in improving a host of biologic applications, for example biomarker discovery, clinical diagnostics, protein interaction, network mapping and many others. Multiplex protein detection and quantification has been constrained by factors including assay specificity, sensitivity, and throughput. Bio-Synthesis offers custom antibody-oligonucleotide conjugates, which permit highly sensitive immunoassay such as immuno-PCR, by immobilizing analytes for each conjugate onto a solid support with cognate capture antibodies. Binding of the antibody-oligonucleotide conjugate to the immobilized analytes allows for amplification of the attached DNA. Products of amplification are visualized using gel electrophoresis, thus denoting the presence of bound analytes. Oligonucleotide-antibody conjugates have been used as suitable tools for high-sensitive protein detection.
Oligonucleotide-Antibody Conjugate Applications
An antigen detection system, in which the polymerase chain reaction (PCR) is used to amplify a segment of marker DNA, has been attached specifically to antigen-antibody complexes. Due to the enormous amplification capability and specificity of PCR, immuno-PCR allows considerable enhancement in the detection sensitivity of a specific antigen over conventional antigen detection systems, such as enzyme-linked immunosorbent assays and radioimmunoassay. A variety of superior characteristics and the versatility of immuno-PCR, including high but controllable sensitivity, simplicity, and easy contamination control, offer great promise for its application in various aspects of biological and medical sciences, as well as clinical diagnostics and forensic medicine.
Multiplexed Assay Development
A planar array or bead-based array, which allows the ability to simultaneously measure in a quantitative manner, multiple analytes of interest across multiple samples using universal capture technology. This technology takes advantage of the specific binding between two complimentary strands of DNA, with one strand placed at a specific location on the microtiter plate. Complementary oligos are conjugated to the user selected antibody. The oligo-antibody conjugates are pooled and hybridized to capture oligos in the well to create an antibody array.
Bio-Synthesis provides high quality antibody-oligonucleotide conjugates using the most advanced bioconjugation chemistry. Additionally, Bio-Synthesis's has skilled scientists, with extensive experience in developing unique bioconjugation strategies for proteins, antibodies, peptides, and oligonucleotides. If you are in need of developing any drug bioconjugates, diagnostic bioconjugates, or research bioconjugates, Bio-Synthesis is here to help you.
Antibody is thiolated with 2-iminothiolane and then reacted with a maleimide-activated oligo.
In this case, sulfhydryl functional groups will be introduced to an antibody through Lys residues using a reliable and reproducible procedure. However, if some of the modified or conjugated Lys residues are located on the antigen binding sites, this method may produce partially active or inactive antibody-enzyme conjugates that may not bind to the antigen. In such a case, an alternative conjugation method may be used.
Quality Control Criteria
The custom product is quality controlled by Bio-Synthesis according to our in-house standards. These are tailored for each individual assignment. The custom conjugate is approved only all quality control criteria have been met.
Our conjugation is based on a mixed short length polymer cross-linked covalent bonded technique. We require ligands with either a primary amine or that are biotinylated.
Due to the nature of each project, a written quotation will be made stating the cost and time required after the receipt of the antibody sample.
Standard pricing covers labor and materials, including the cost of maleimide-activated oligo preparation, antibody thiolation, conjugation procedure, conjugate purification and analysis.
Conjugations take 1-2 weeks. All shipments are sent Fed Ex Standard Overnight delivery.
*No shipments on Fridays
Sample Submission Guideline
As a rule, Bio-Synthesis will only accept pure substances as starting material, either lyophilized or dissolved in a medium of known chemical composition. Interfering substances must not be present. Non-commercial antibodies supplied by the customer should be affinity purified. Please provide gel electrophoresis data along with your antibody. Commercially available antibodies can be supplied by customers or ordered through Bio-Synthesis. The cost of acquiring antibodies will be added to the invoice, plus an administration charge (15% of total cost). We can assist in antibody modification or fragmentation prior to any immobilization reaction.